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Hum. Reprod. Advance Access originally published online on February 27, 2008
Human Reproduction 2008 23(4):934-943; doi:10.1093/humrep/den051
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells

K.E. Schwab1, P. Hutchinson2 and C.E. Gargett1,3

1 Centre for Women's Health Research, Monash Institute of Medical Research and Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, Clayton, Victoria, Australia 2 Centre for Inflammatory Diseases, Monash University, Monash Medical Centre, Clayton, Victoria, Australia

3 Correspondence address. Tel: +61-3-9594-5392; Fax: +61-3-9594-6389; E-mail: caroline.gargett{at}med.monash.edu.au

BACKGROUND: Human endometrium is a highly regenerative tissue. We hypothesized that the source of endometrial stromal and vascular regeneration is a resident stromal stem/progenitor cell population. Putative human endometrial stromal stem/progenitor cells have been identified using clonal assays, a retrospective functional stem cell assay. Therefore, the aim of this study was to screen potential stem cell markers for the prospective isolation of human endometrial stromal stem/progenitor cells and to determine their capacity to identify colony-forming stromal cells.

METHODS: Single-cell suspensions of human endometrial stromal cells were sorted using fluorescence-activated cell sorting into positive and negative populations based on STRO-1, CD133, CD90 or CD146 expression for clonal assays. All markers were immunolocalized in human endometrium.

RESULTS: Small populations (2–9%) of human endometrial stromal cells expressed each of the markers. Only CD146+ cells were enriched for colony-forming cells, and CD90hi cells showed a trend for greater enrichment compared with CD90lo cells. STRO-1 and CD146 were localized to perivascular cells of the endometrium. CD90 was strongly expressed by functionalis stroma and perivascular cells, but only weakly expressed in the basalis stroma. CD133 was expressed by epithelial cells of the endometrium, rather than by stroma or perivascular cells.

CONCLUSIONS: This study identified CD146 as a marker of colony-forming human endometrial stromal cells supporting the concept that human endometrium contains a population of candidate stromal stem/progenitor cells.

Key words: human endometrium/stromal stem cells/cell surface markers/clonal assays

Submitted on October 10, 2007; resubmitted on January 31, 2008; accepted on February 4, 2008.


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