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Hum. Reprod. Advance Access originally published online on March 19, 2008
Human Reproduction 2008 23(5):1063-1068; doi:10.1093/humrep/den078
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis

J.R.A. Sherwin1, A.M. Sharkey2, A. Mihalyi3, P. Simsa3, R.D. Catalano2 and T.M. D'Hooghe3,4

1 Department of Obstetrics and Gynaecology, The Rosie Hospital, Robinson Way, Cambridge CB2 2SW, UK 2 Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK 3 Faculty of Medicine, Department of Obstetrics and Gynecology, Leuven University Fertility Center, UZ Gasthuisberg, 3000 Leuven, Belgium

4 Correspondence address. Tel: +32 16 343624; Fax: +32 16 344368; E-mail: thomas.dhooghe{at}uz.kuleuven.ac.be

BACKGROUND: Endometriosis occurs in 10% of women and is currently diagnosed by invasive laparoscopic testing. We tested the hypothesis that endometrial gene expression in late secretory phase endometrium differs between patients with and without endometriosis.

METHODS: Ten patients with laparoscopically proven endometriosis (minimal/mild n = 5 and moderate/severe n = 5) and six controls, underwent endometrial biopsy in the late secretory phase (Day 23 onwards). Microarray interrogation of eutopic endometrial gene expression was performed.

RESULTS: Microarray data were obtained for all control samples and eight samples from the endometriosis patients (n = 4 minimal/mild, n = 4 moderate/severe disease). Eight genes were identified as up-regulated and one gene was down-regulated in all endometriotic samples (more than 1.75-fold, P < 0.01). Real-time PCR analysis of protocadherin-17 (PCDH17), protein tyrosine phosphatase, receptor type, R (PTPRR) and interleukin-6 signal transducer (IL6ST) expression validated the microarray findings.

CONCLUSIONS: Expression of very few transcripts differs, in late secretory eutopic endometrium, between controls and patients with endometriosis. The median fold changes of these genes are small. No transcripts were identified that could discriminate between minimal/mild and moderate/severe endometriosis. Therefore, interrogation of the late secretory endometrial transcriptome is not likely to form the basis of a minimally invasive diagnostic test for endometriosis.

Key words: endometrium/endometriosis/protocadherin-17/protein tyrosine phosphatase, receptor type, R/microarray

Submitted on May 13, 2007; resubmitted on February 11, 2008; accepted on February 22, 2008.


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