Skip Navigation


Hum. Reprod. Advance Access originally published online on June 26, 2009
Human Reproduction 2009 24(10):2457-2467; doi:10.1093/humrep/dep117
This Article
Right arrow Full Text
Right arrow Full Text (PDF )
Right arrow All Versions of this Article:
24/10/2457    most recent
dep117v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Hum. Reprod.
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bielanski, A.
Right arrow Articles by Vajta, G.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bielanski, A.
Right arrow Articles by Vajta, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units

A. Bielanski1,4 and G. Vajta2,3

1 Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa/Nepean, ON, Canada K2H 8P9 2 PIVET Medical Centre, 166-168 Cambridge Street, Perth, WA 6007, Australia 3 Cairns Fertility Centre, Applin and McLeod Corner, Cairns, QLD 4870, Australia

4 Correspondence address. Tel: +1-613-228-6698; E-mail: bielanskia{at}inspection.gc.ca

Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies. In parallel, concerns are increasing about the technical and biological safety of these procedures. However, published data regarding the confirmed or theoretical hazards of these procedures are sparse and sometimes contradictory. The purpose of this review will summarize data and opinions about one of the most disputed risks, the potential hazard of contamination and disease transmission through cryopreservation. Special attention is concentrated on the weak points of the technology including open vitrification systems, sterilization of liquid nitrogen and safety of commonly used storage tanks including straws and cryovials. Suggestions are also made for practical measures to avoid these dangers while preserving the benefits and perspectives of new cryopreservation technologies.

Key words: cryopreservation/vitrification/freezing/contamination/liquid nitrogen

Submitted on December 16, 2008; resubmitted on March 30, 2009; accepted on April 6, 2009.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?

Related articles in Hum. Reprod.:

Editor's Choice
Andre Van Steirteghem
Hum. Reprod. 2009 24: 2387-2388. [Extract] [Full Text]  





Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.