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Hum. Reprod. Advance Access originally published online on July 22, 2009
Human Reproduction 2009 24(11):2868-2878; doi:10.1093/humrep/dep263
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© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

LH/hCGR gene expression in human cumulus cells is linked to the expression of the extracellular matrix modifying gene TNFAIP6 and to serum estradiol levels on day of hCG administration

D. Haouzi1,2,3, S. Assou1,2,3, K. Mahmoud4, B. Hedon2, J. De Vos1,3, D. Dewailly5 and S. Hamamah1,2,3,6

1 Institut de Recherche en Biothérapie, CHU Montpellier, Université Montpellier I, Hôpital Saint-Eloi, Montpellier F-34000, France 2 Département de Médecine et Biologie de la Reproduction, CHU Montpellier, Hôpital Arnaud de Villeneuve, Montpellier F-34000, France 3 INSERM, U847 ‘Développement embryonnaire précoce et cellules souches embryonnaires humaines’, Montpellier F-34000, France 4 Centre de FIV, Clinique les Jardins, Tunis, Tunisia 5 Département de Gynécologie Endocrinienne et Médecine de la Reproduction, CHU Lille, Hôpital Jeanne de Flandre, Lille F-59037, France

6 Correspondence address. ART/PGD Division, Department of Reproductive Medicine, Arnaud de Villeneuve Hospital, 34295 Montpellier, France. Tel: +33-4-67-33-64-04; Fax: +33-4-67-33-62-90; E-mail: s-hamamah{at}chu-montpellier.fr

BACKGROUND: Recent studies suggest a role for luteinizing hormone and human chorionic gonadotrophin receptor (LH/hCGR) signalling in the regulation of the oocyte–cumulus oophorus cell interplay. The present study aimed at assessing the LH/hCGR gene expression in cumulus cells (CCs) surrounding oocytes in patients undergoing controlled ovarian hyperstimulation (COS) before ICSI and to relate the LH/hCGR expression to other COS quality parameters.

METHODS: CCs from single oocytes of normal responder patients were analysed by DNA microarrays. Concomitantly, estradiol levels on the day of hCG administration, CC morphology, total collected oocyte and metaphase II oocyte number were assessed in relation to LH/hCGR gene expression in CC.

RESULTS: The transcriptome analysis of CC indicated a variable expression of LH/hCGR among the patients and intra-patients. LH/hCGR mRNA expression was negatively correlated with serum estradiol level on the day of hCG administration. Eighty-five genes were significantly modulated between CCs from patients with a high and a low LH/hCGR expression. These genes are involved principally in steroid metabolism and in the ovulation process and include TNFAIP6, a gene expressed during CC–oocyte complex (COC) expansion. There were no significant differences in LH/hCGR gene expression profile between COS protocols.

CONCLUSIONS: LH/hCGR is expressed in CC under COS conditions. LH/hCGR expression level is associated with TNFAIP6 gene expression and negatively correlated with serum estradiol level on the day of hCG administration.

Key words: cumulus cells/LH/hCGR/TNFAIP6 gene

Submitted on November 14, 2008; resubmitted on June 22, 2009; accepted on June 26, 2009.


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