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Hum. Reprod. Advance Access originally published online on December 3, 2008
Human Reproduction 2009 24(2):308-314; doi:10.1093/humrep/den432
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Viability of bovine embryos following exposure to the green filtered or wider bandwidth light during in vitro embryo production

Kati Korhonen1,6, Sami Sjövall2,5, Jouko Viitanen2, Elise Ketoja3, Alexander Makarevich4 and Jaana Peippo1

1 MTT Agrifood Research Finland, Biotechnology and Food Research, Animal Genomics FI-31600, Jokioinen, Finland 2 VTT Technical Research Centre of Finland FI-33101, Tampere, Finland 3 MTT Agrifood Research Finland, Services Unit FI-31600, Jokioinen, Finland 4 Slovak Agricultural Research Centre, Research Institute of Animal Production 94992, Nitra, Slovak Republic

6 Correspondence address. Tel: +358-3-4188-3684; Fax: +358-3-4188-3618; E-mail: kati.korhonen{at}mtt.fi/kati.korhonen{at}surffi.net

BACKGROUND: Microscopic light during in vitro production (IVP) is a common stress factor compromising embryo development and viability. Many studies discussing detrimental effects of light have been conducted on in vivo matured/fertilized oocytes or on flushed embryos that were exposed to light only when cultured in vitro. The aim of this work was to examine the effects of light composition during all IVP steps on subsequent embryo development and quality.

METHODS: We compared the effects of a green pass filter of 498–563 nm wavelength, and a wider bandwidth of stereomicroscopic light on bovine embryo development rates, total cell counts and the presence of constitutive (Hsp73) and stress-inducible (Hsp72) forms of the Hsp70 protein.

RESULTS: The use of the green filter had no effect on embryo development rates, morphological quality or total cell counts on Day 7 or 8 of development compared with control group. However, Hsp72/73 protein levels revealed the protective effect of the filter against harmful blue and infrared regions of the light. The constitutive form Hsp73 was seen in both groups, but the inducible stress-response form Hsp72 was absent from the filter group embryos and appeared only in the group exposed to the stereomicroscopic light.

CONCLUSIONS: An easy to use and inexpensive green filter seems to reduce the stress caused by light during the IVP procedures without affecting either the accuracy of embryo monitoring or the need to increase the light intensity.

Key words: IVM/IVF/IVC/light-induced stress/Hsp70/green filter

5 Present address: Plenware Oy Ltd, Pakkahuoneenaukio 2 A, FI-33100, Tampere, Finland

Submitted on July 24, 2008; resubmitted on October 17, 2008; accepted on October 29, 2008.


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