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Hum. Reprod. Advance Access originally published online on December 23, 2008
Human Reproduction 2009 24(4):805-814; doi:10.1093/humrep/den388
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Change in gene expression of mouse embryonic stem cells derived from parthenogenetic activation

Seung Pyo Gong1, Heebal Kim1,5, Eun Ju Lee2, Seung Tae Lee3, Sunjin Moon1, Ho-Joon Lee4 and Jeong Mook Lim1,5,6

1 Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea 2 Clinical Research Institute, Seoul National University Hospital, Seoul 110-744, Korea 3 Laboratory of Regenerative Medicine and Pharmacology, Integrative Biosciences Institute, Swiss Federal Institute of Technology at Lausanne, Lausanne, Switzerland 4 Vincent Center for Reproductive Biology, Massachusetts General Hospital, Harvard University Medical School, Boston MA02114, MA, USA 5 Research Institute for Agriculture and Life Science, Seoul National University, Seoul 151-742, Korea

6 Correspondence address. Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Building 200-#4223, Sillim-9 Dong, Seoul 151-921, Korea. Fax: +822-874-2555; E-mail: limjm{at}snu.ac.kr

BACKGROUND: We previously established parthenogenetic mouse embryonic stem cells (ESCs) and this study was subsequently conducted for elucidating the influence of oocyte parthenogenesis on gene expression profile of ESCs.

METHODS: Gene expression of parthenogenetic ESC (pESC)-1 or pESC-2 was separately compared with that of two normally fertilized ESC (nfESC) lines (B6D2F1 and R1 strains), and quantification of mRNA expression was conducted for validating microarray data.

RESULTS: In two sets of comparison, reaction of 11 347 and 15 454 gene probes were altered by parthenogenesis, while strain difference changed the expression of 15 750 and 14 944 probes. Level of correlation coefficient was higher in the comparisons between normal fertilization and parthenogenesis (0.974–0.985) than in the comparisons between strains of nfESCs (0.97–0.971). Overall, the expression of 3276–3329 genes was changed after parthenogenesis, and 88% (96/109) of major functional genes differentially (P < 0.01) expressed in one comparison set showed the same change in the other. When we monitored imprinted genes, expression of nine paternal and eight maternal genes were altered after parthenogenesis and 88% (14/16) of these was confirmed by mRNA quantification.

CONCLUSIONS: The change in gene expression after parthenogenesis was similar to, or less than, the change induced by a strain difference under a certain genetic background. These results may suggest the clinical feasibility of parthenogenesis-derived, pluripotent cells.

Key words: mouse model/embryonic stem cell/normal fertilization/parthenogenesis/gene expression

Submitted on February 19, 2008; resubmitted on September 25, 2008; accepted on October 7, 2008.


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