Skip Navigation


Hum. Reprod. Advance Access originally published online on April 29, 2009
Human Reproduction 2009 24(8):2034-2043; doi:10.1093/humrep/dep118
This Article
Right arrow Full Text
Right arrow Full Text (PDF )
Right arrow All Versions of this Article:
24/8/2034    most recent
dep118v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Pinton, A.
Right arrow Articles by Ducos, A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pinton, A.
Right arrow Articles by Ducos, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Influence of sex on the meiotic segregation of a t(13;17) Robertsonian translocation: a case study in the pig

A. Pinton1,3, A. Calgaro1, N. Bonnet1, S. Ferchaud2, S. Billoux1, A.M. Dudez1, N. Mary1, K. Massip1, A. Bonnet-Garnier1, M. Yerle1 and A. Ducos1

1 INRA-ENVT, UMR 444 Génétique Cellulaire, F-31076 Toulouse, France 2 INRA/UEICP, F-86480 Rouillé, France

3 Correspondence address. E-mail: a.pinton{at}envt.fr

BACKGROUND: Comparison of male versus female meiotic segregation patterns for Robertsonian translocation (RT) carriers with similar genetic background has rarely been reported in mammalian species.

METHODS: The aim of this study was to compare the segregation patterns determined for related males and females carrying a 13;17 RT in an animal model (Sus scrofa domestica L.), using dual colour fluorescence in situ hybridization on decondensed sperm nuclei and metaphases II of in vitro-matured oocytes.

RESULTS: In males, no association between the trivalent and the XY body was observed in any of the 90 pachytene nuclei studied, and the rate of unbalanced spermatozoa ranged between 2.96% and 3.83%. Female meiotic segregation analyses were carried out on 83 metaphase II oocytes. The rate of unbalanced gametes was higher in females than in males (28.91% versus 3.21%, P < 0.001). This difference was due to higher rates of diploid gametes (12.04% versus 0.05%) and unbalanced gametes produced by the adjacent segregation (16.86% versus 3.16%).

CONCLUSIONS: This study is a new scientific contribution to the comparison of segregation patterns in related males and females carrying an identical chromosomal rearrangement. It allows a better understanding of the meiotic behaviour of RTs. It also clearly illustrates the relevance of swine as an animal model for such meiotic studies.

Key words: chromosome pairing/meiosis/pig/Robertsonian translocation/segregation

Submitted on January 7, 2009; resubmitted on March 27, 2009; accepted on April 7, 2009.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.