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Human Reproduction, Vol. 3, No. 3, pp. 377-381, 1988
© 1988 European Society of Human Reproduction and Embryology

research-article

Pre-implantation diagnosis of HPRT-deficient male and carrier female mouse embryos by trophectoderm biopsy

M. Monk1,2, A.L. Muggleton-Harris, E. Rawlings and D.G. Whittingham

MRC Experimental Embryology and Teratology Unit, Medical Research Council Laboratories Woodmansterne Road, Carshalton, Surrey SM5 4EF 1MRC Mammalian Development Unit, University College London 4 Stephenson Way, London NW1 2HE, UK

Correspondence: 2To whom correspondence should be addressed

In an animal model for Lesch-Nyhan syndrome, the affected male embryos, as well as the carrier female embryos, have been successfully identified by biochemical microassay of a sample of trophectoderm cells taken from the mouse embryos at the blastocyst stage. The embryos were removed from the uterus, diagnosed and returned to the uterus within 2 days without the need for cryopreservation. The diagnosis was con firmed at 14 days gestation by analysis of the hypoxanthine phosphoribosyl transferase (HPRT) status of the fetuses. Live young were obtained from biopsied embryos after transfer.

Key words: pre-implantalion/diagnosis/Lesch-Nyhan syndrome/trophectoderm/biopsy


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