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Human Reproduction, Vol. 3, No. 8, pp. 1004-1009, 1988
© 1988 European Society of Human Reproduction and Embryology

research-article

Microassay for adenosine deaminase, the enzyme lacking in some forms of immunodeficiency, in mouse preimplantation embryos

Cathy Benson and Marilyn Monk

MRC Mammalian Development Unit, Wolfson House, University College London 4 Stephenson Way, London NW1 2HE, UK

A highly sensitive biochemical microassay has been developed for adenosine deaminase (ADA; EC 3.5.4.4), the enzyme deficient in ~20% of cases of severe combined immunodeficiency disease (SCID). The microassay is capable of detecting femtomolar amounts of reaction product in a single blastomere from a mouse 8-cell embryo and thus is sensitive enough to be considered for the possible prehnplantation diagnosis of SCID in human embryos.

Key words: adenosine deaminase/microassay/preimplantation diagnosis/severe combined immunodeficiency disease/mouse


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