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Human Reproduction, Vol. 3, No. 8, pp. 960-967, 1988
© 1988 European Society of Human Reproduction and Embryology

research-article

Effect of 1,2-propanediol and dimethylsulphoxide on the meiotic spindle of the mouse oocyte

Josiane Van der Elst, Etienne Van den Abbeel, Roland Jacobs1, Eddie Wisse1 and André Van Steirteghem

Centre for Reproductive Medicine Vrije Universiteit Brussel, Medical Campus Laarbeeklaan 103, 1090 Brussels, Belgium 1Laboratoty for Cell Biology and Histology, Vrije Universiteit Brussel, Medical Campus Laarbeeldaan 103, 1090 Brussels, Belgium

At ovulation, the mouse oocyte is arrested at metaphase of the second meiotic division. Since microtubules are thermo-and chemosensitive structures, the effects of 1.5 M dlmethylsulphoxide and 1.5 M 1,2-propanediol were studied at room temperature on the morphology of the meiotic spindle. Oocytes incubated at 37°C or at room temperature served to estimate the effect of temperature in the experiment. The meiotic spindle was visualized by immunogold-silver staining of microtubules. In the control group at 37°C, 88% of oocytes had normal spindles. After incubation at room temperature for the same time, 89% of oocytes showed abnormal spindles. In the oocytes exposed to dimethyl sulphoxide or 1,2-propanediol at room temperature a protective effect on spindle morphology could be recognized. Subsequent incubation at 37°C resulted in partial restoration of the observed abnormalities after cooling to room temperature and after exposure to dhnethytsulphoxide. incubation at 37°C after exposure to 1,2-propanediol at room temperature induced spindle absence in the majority of oocytes. Although this latter condition allowed fertilization without increased incidence of ploidy abnormalities, a role for 1 as an activating agent is hypothesized.

Key words: dimethylsulphoxide/IVF/meiotic spindle/mouse oocyte/1,2-propanediol


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