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Human Reproduction, Vol. 3, No. 8, pp. 990-992, 1988
© 1988 European Society of Human Reproduction and Embryology

research-article

A simplified method for fixation of human and mouse preimplantation embryos which facilitates G-banding and karyotypic analysis

C.G. Roberts and C. O'Neill

Human Reproduction Unit, Royal North Shore Hospital St Leonards, N.S.W. 2065, Australia

A reliable method for the fixation of large numbers of prelmplantatlon embryos was developed to allow application of routine G-banding methods to preimplantatlon stage embryos. The technique resulted in minimal loss of embryos and was suitable for both 1-cell mouse embryos and multipronuclear human embryos. Application of this method will increase the information gained from chromosome studies of embryonic development.

Key words: IVF/Preimplantation diagnosis/G-banding/fixation


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