Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (85)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Collier, M.
Right arrow Articles by Saunders, D.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collier, M.
Right arrow Articles by Saunders, D.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 3, No. 8, pp. 993-998, 1988
© 1988 European Society of Human Reproduction and Embryology

research-article

Biochemical and pharmacological characterization of human embryo-derived platelet activating factor

M. Collier, C. O'Neill, A.J. Ammit and D.M. Saunders

Human Reproduction Unit, Royal North Shore Hospital St Leonards, NSW 2065, Australia

The soluble platelet activating factor (PAF) produced by mouse embryos was shown to have properties similar to 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine (PAF-acether). In this study PAF was extracted from the medium In which human embryos were cultured for ~ 18 h prior to transfer. The extracted embryo-derived PAF moved on silica thin layer chromatograms with the same RF of 0.26 ± 0.03 (n = 26) as PAF-acether. Embryo-derived PAF or PAF-acether activity was assayed by monitoring the decrease in the proportion of single platelets in rabbit whole blood due to aggregation on incubation at 37°C. The two agonists were said to be of the same activity, if they induced the same degree of platelet aggregation after 15 min incubation. PAF-acether (93 nM) and embryo-derived PAF of similar activity induced an Identical time response of platelet aggregation, the response being maximal by 6 min. PAF-acether, over the range 5.6–200 nM, induced a decrease that was linear when plotted on a log-log scale. Embryo-derived PAF and PAF-acether (184 nM) gave identical dose responses when serially diluted to 16 nM. Pharmacologically, the action of embryo-derived PAF and PAF-acether (46 nM) on platelet aggregation was significantly inhibited by 3.75 µM of the PAF-speeific receptor inhibitor, SRI 63-441, and completely inhibited at 15 µM SRI 63-441. Embryo-derived PAF and PAF-acether (184 nM) were inactivated to the same degree by incubation with 5–13 IU/ml phospholipase A2 (pA2) Thus, the embryo-derived PAF from the human was shown on the basis of biochemical and pharmacological characterization to be homologous to PAF-acether.

Key words: platelet activating factor (PAF)/PAF-acether


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
C. O'Neill
The potential roles for embryotrophic ligands in preimplantation embryo development
Hum. Reprod. Update, May 1, 2008; 14(3): 275 - 288.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y. Li, V. Chandrakanthan, M. L Day, and C. O'Neill
Direct Evidence for the Action of Phosphatidylinositol (3,4,5)-Trisphosphate-Mediated Signal Transduction in the 2-Cell Mouse Embryo
Biol Reprod, November 1, 2007; 77(5): 813 - 821.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
N. Gopichandran and H. J Leese
The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos
Reproduction, February 1, 2006; 131(2): 269 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
C. O'Neill
The role of paf in embryo physiology
Hum. Reprod. Update, May 1, 2005; 11(3): 215 - 228.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. H. Lee, D. Y. Kim, D. H. Nam, S. H. Hyun, G. S. Lee, H. S. Kim, C.-K. Lee, S. K. Kang, B. C. Lee, and W. S. Hwang
Role of Messenger RNA Expression of Platelet Activating Factor and Its Receptor in Porcine In Vitro-Fertilized and Cloned Embryo Development
Biol Reprod, September 1, 2004; 71(3): 919 - 925.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. P. Lu, Y. Li, R. Bathgate, M. Day, and C. O'Neill
Ligand-Activated Signal Transduction in the 2-Cell Embryo
Biol Reprod, July 1, 2003; 69(1): 106 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
W.E. Roudebush, J.D. Wininger, A.E. Jones, G. Wright, A.A. Toledo, H.I. Kort, J.B. Massey, and D.B. Shapiro
Embryonic platelet-activating factor: an indicator of embryo viability
Hum. Reprod., May 1, 2002; 17(5): 1306 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T. Stojanov and C. O'Neill
In Vitro Fertilization Causes Epigenetic Modifications to the Onset of Gene Expression from the Zygotic Genome in Mice
Biol Reprod, February 1, 2001; 64(2): 696 - 705.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
T. Stojanov, S. Alechna, and C. O'Neill
In-vitro fertilization and culture of mouse embryos in vitro significantly retards the onset of insulin-like growth factor-II expression from the zygotic genome
Mol. Hum. Reprod., February 1, 1999; 5(2): 116 - 124.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.