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Human Reproduction, Vol. 4, No. 6, pp. 702-705, 1989
© 1989 European Society of Human Reproduction and Embryology

other

The use of Albuminar 5 (TM) as a medium supplement in clinical FVF

M. J. Ashwood-Smith3, P. Hollands1 and R.G. Edwards2

Bourn Hall Clinic Bourn, Cambridge CB3 7TR, UK

Correspondence: 3Department of Biology, University of Victoria, PO Box 1700, Victoria, BC V8W 2Y2, Canada

Human serum and Albuminar 5 (A5) were compared as medium supplements to Earle's solution containing pyruvate in clinical IVF. One-hundred patients in each group showed a fertilization rate of 60% with serum and of 62% with A5. The overall pregnancy rates in the serum and A5 groups were 20 and 24%, respectively. The incidence of failed fertilization (6–7%) and of multipronucleate oocytes (4–5%) was similar in both groups. At 37°C, sperm survived less well in A5 although the rate of fertilization was not reduced. Blastocyst formation was not seen in ‘spare’ embryos grown in vitro in medium containing 15% v/v A5.

Key words: IVF/media/serum albumin

1Present address: Department of Biology, University of Victoria, PO Box 1700, Victoria, BC V8W 2Y2, Canada

2Present address: The Grange IVF Unit, Bottisham, Cambridge CB5 9BD, UK


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