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Human Reproduction, Vol. 4, No. suppl_1, pp. 73-77, 1989
© 1989 European Society of Human Reproduction and Embryology

Minimal andrological requirements for in-vitro fertilization

H.-H. Riedel, F. Hübner, S. C. Ensslen, K. W. Bieniek and M. Grillo1

Department of Obstetrics and Gynaecology RWTH Aachen, RWTH Aachen, Medical Faculty, D-5100 Aachen 1 Department of Obstetrics and Gynaecology University of Kiel Medical Faculty, D-2300 Kiel, FRG

The ejaculate used for in-vitro fertilization of human oocytes must fulfil minimum requirements. Based on the results of experimental investigations, total concentrations of spermatozoa must be >5 million spermatozoa/µl with progressive motilities of at least 30% and also a normal morphology of at least 30%. Furthermore, in sperm preparations used for inseminating human oocytes, the concentration of pathogenic microorganisms must not be <104. If there are positive bacteriological findings, the treatment of both partners is mandatory according to the antibiogram. According to the information gathered to date, the hamster oocyte penetration test can only provide additional information if it is positive, but acceptance into an IVF programme should not depend on this test. According to the results presented so far, the swell test developed by Jeyendran shows quite good correlation with the fertilization of human oocytes.

Key words: andrological factors in IVF/mouse-oocyte system/sperm bacteriology HOS and HOP test/treatment of positive sperm bacteriology


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