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Human Reproduction, Vol. 5, No. 1, pp. 14-18, 1990
© 1990 European Society of Human Reproduction and Embryology

research-article

Identification of specific serum proteins synthesized de novo by monolayer cultures of glandular cells of gestational endometrium

T.N. Fay, S. Lindenberg1, B. Teisner2, L.G. Westergaard1, J.G. Westergaard3 and J.G. Grudzinskas4

Academic Unit of Obstetrics and Gynaecology, London Hospital Medical College Whitechapel, London El, UK 1Department OB/GYN, Rigshopitalet, University of Copenhagen Odense, Denmark 2institute of Medical Microbiology Odense, Denmark 3Department of Obstetrics and Gynaecology, Odense University Odense, Denmark

Correspondence: 4To whom correspondence should be addressed

Monolayer cell cultures (n = 3) of glandular epithelium of gestational endometrium obtained from three apparently healthy women undergoing elective termination of pregnancy (7–9 weeks gestation) were established. De novo synthesis of eight serum proteins (albumin, alpha1-antitrypsin, cerulo-plasmin, beta-lipoprotein, alpha2-macroglobulin, fibronectin and complement factors C3 and C4) was demonstrated by the incorporation of radiolabelled substrate ([35-S]methionine) employing autoradiography (AR) in combination with crossed immunoelectrophoresis (XIE), referred to as ARXIE, and line immunoelectrophoresis (LIE), referred to as ARLIE. By contrast, there was no evidence for de novo synthesis of IgA, haptoglobin and orosomucoid. Our findings suggest that the gestational endometrium may contribute to the production of several proteins considered to be synthesized and secreted mainly by the liver and reticulo-endothelial system. The simple techniques used here to identify the de novo synthesis of human serum proteins could be applied to investigate protein synthesis by a wide range of tissues and cells

Key words: autoradiography/gestational endometrium/glandularepithelium/immunoelectrophoresis/serum proteins


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