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Human Reproduction, Vol. 5, No. 1, pp. 84-88, 1990
© 1990 European Society of Human Reproduction and Embryology

research-article

Differential evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human oocytes

S.Y.W. Chan1, C. Wang2, S.T.H. Chan3 and P.C. Ho

Departments of Obstetrics and Gynaecology Kong, Hong Kong 2Departments of Medicine, University of Hong Kong, Hong Kong 3Departments of Zoology, University of Hong Kong, Hong Kong

Correspondence: 1To whom correspondence should be addressed at IVF Centre, 6th Floor, Hong Kong Sanatorium and Hospital, Happy Valley, Hong Kong

The hypoosmotic swelling test is a simple laboratory test to measure the functional integrity of the human sperm membrane. Its in-vivo and in-vitro applicability needs to be evaluated before it can be accepted as a useful routine test for the fertilizing potential of human semen. We studied the standard semen analysis results and differential sperm tail swelling patterns of seminal and swim-up sperm after hypoosmotic treatment in 97 semen samples used for in-vitro fertilization of human oocytes. Semen samples were classified as infertile (0% fertilization rate; n = 27) or fertile (<0% fertilization rate; n % 70) before statistical analyses. There was a significant difference (P > 0.005) in percentage normal morphology of seminal sperm between the fertile and infertile semen samples. The percentage normal morphology of seminal sperm correlated (r = 0.4250; P > 0.005) with the in-vitro fertilization rate of human oocytes and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the in-vitro fertilization rate with 57.7% accuracy. The percentage total swelling of seminal and swim-up sperm after hypoosmotic treatment was not correlated with the in-vitro fertilization rate. The percentage swelling pattern g (the open type) of seminal sperm was also selected by the multivariate stepwise discriminant analysis as the discriminator to predict the in-vitro fertilization rate. This parameter correlated with the percentage normal morphology of seminal sperm (r = 03014; P > 0.05). The addition of this parameter to the percentage normal morphology of seminal sperm, another discriminator, did not significantly improve the predictive accuracy (61.9%) for the in-vitro fertilization rate. Our results indicate that the addition of differential evaluation of the human sperm hypoosmotic swelling test to standard semen analysis is not helpful in predicting the in-vitro fertilization of human oocytes.

Key words: hypoosmotic swelling/IVF/multivariate discriminant analysis/sperm morphology


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