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Human Reproduction, Vol. 5, No. 8, pp. 928-932, 1990
© 1990 European Society of Human Reproduction and Embryology

other

Effect of clomiphene isomers on oestradiol synthesis in cultured human granulosa cells

J.-H. Olsson and S. Granberg

Department of Obstetrics and Gynaecology, University of Göteborg S-413 45 Göteborg, Sweden

The effects of en-clomiphene and zu-clomiphene (10–7–10–5 M) on oestradiol synthesis in human granulosa cells were studied. Granulosa cells were obtained from stimulated cycles in women undergoing oocyte retrieval, in the course of in-vitro fertilization/embryo transfer due to mechanical infertility. En-clomiphene and zu-clomiphene (10–5 M) significantly reduced basal and LH-stimulated oestradiol synthesis after 2–4 days of culture. The LH-stimulated synthesis was also significantly and reversibly reduced by zu-clomiphene at lower concentrations (10–6–10–7 M) and by a mixture of en- and zu-clomiphene (10–6 M). It is concluded that both the mixture of en- and zu-clomiphene and the isomers alone have direct inhibitory effects on oestradiol synthesis in human granulosa cells in vitro. Furthermore, it cannot be excluded that the LH-stimulated oestradiol synthesis is inhibited by zu-clomiphene at concentrations found in follicular fluid after administration of clomiphene citrate.

Key words: clomiphene citrate/isomers/human/ovary/granulosa cells/steroidogenesis


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