Human Reproduction, Vol. 5, No. 8, pp. 997-1003, 1990
© 1990 European Society of Human Reproduction and Embryology
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The effect of iron and iron chelators on the in-vitro block to development of the mouse preimplantation embryo: BAT6 a new medium for improved culture of mouse embryos in vitro
Department of Anatomy Downing Street, Cambridge CB2 3DY 2MRC Reproductive Biology Unit, Centre for Reproductive Biology 37 Chalmers Street, Edinburgh EH3 9EW, UK
Correspondence: 1To whom correspondence should be addressed
The effect of iron and iron chelators on the development of the mouse embryo in vitro from the 1-cell stage to the blastocyst has been investigated. An adverse effect of iron was found. The high affinity iron chelator, desferal, also blocked development, whilst transferrin (whether as apoprotein or saturated with iron), DETAPAC and EDTA promoted development. The addition of transferrin permitted development to the blastocyst stage of embryos from strains normally exhibiting the 2-cell block. Under such circumstances both the rate of embryonic development and the proportion of embryos reaching the blastocyst stage approached levels found in vivo. Based on these results, a new medium, BAT6, is described for the optimal in-vitro culture of mouse embryos.
Key words: iron/transferrin/in-vitro block/mouse embryo
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