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Human Reproduction, Vol. 6, No. 10, pp. 1445-1448, 1991
© 1991 European Society of Human Reproduction and Embryology

other

Block to development in cultured rat 1-cell embryos is overcome using medium HECM-1

Junji Kishi1, Yoichi Noda, Katsuhiko Narimoto, Yoh Umaoka and Takahide Mori

Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University 54 Shogoinkawahara-cho, Sakyo-ku, Kyoto 606-01 Japan

Correspondence: 1To whom correspondence should be addressed

Rat pronuclear embryos were cultured in hamster embryo culture medium-1 (HECM-1) or a modified Krebs-Ringer bicarbonate solution (mKRB). Embryo cultures in HECM-1 were also challenged with low oxygen concentrations. In HECM-1, 57.9% (70/121) of the pronuclear embryos developed into the 4-cell stage after 48 h of culture. The rates of 8-cell, morula and blastocyst formation were 32.2% (39/121), 17.4% (21/121) and 9.9% (12/121), respectively. On the other hand, in mKRB, rat pronuclear embryos showed developmental blockages at the 2-cell and 4-cell stages, and never developed beyond the 4-cell stage. The rate of blastocyst formation under a low oxygen concentration was 20.1% (43/214), showing a significant difference from the value of 5.5% (11/201) obtained under a standard oxygen concentration (P < 0.005). This is the first report of successful culture of rat pronuclear embryos to the blastocyst stage. Furthermore, it is suggested that protection from oxidation stress is a prerequisite for rat embryo development in vitro.

Key words: developmental blockage/glucose/oxygen/phosphate/rat embryo


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