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Human Reproduction, Vol. 6, No. 5, pp. 623-631, 1991
© 1991 European Society of Human Reproduction and Embryology

other

Quality of oocytes from superovulated rhesus monkeys

Lorna D. Johnson1, Britta A. Mattson3, David F. Albertini3, Prabhat K. Sehgal, Robert A. Becker, Jacqueline Avis4 and John D. Biggers2

New England Regional Primate Research Center (NERPRC), Harvard Medical School Southborough, MA 01772 3Laboratory for Human Reproduction and Reproductive Biology (LHRRB), and Department of Physiology, Harvard Medical School Boston, MA 02115 4Department of Anatomy and Cellular Biology, Tufts University Health Science Center Boston, MA 02111 2La Jolla Cancer Institute La Jolla, CA, USA

Correspondence: 2To whom correspondence should be addressed

The parameters, serum oestradiol (E2) response, follicle size and cumulus morphology, which are commonly used to determine in-vivo oocyte maturation in human in-vitro fertilization and embryo transfer (IVF-ET) programmes, were shown to be unreliable predictors of maturation of rhesus oocytes. In two groups of rhesus, one stimulated with pregnant mares' serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) and the other with human menopausal gonadotrophin (HMG) and HCG, the three parameters varied widely within and between protocols. Triple fluorochrome staining (TFS) for chromatin, microtubules and filamentous actin (f-actin) in oocytes at the time of collection and following 24 h in culture showed major differences in their maturation both in vivo and in vitro following priming with PMSG and HMG. In evaluating IVF protocols, TFS provides a valuable assay for the meiotic status of fixed oocytes of non-human primates.

Key words: oocyte maturation/rhesus/superovulation


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