Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Munne, S.
Right arrow Articles by Estop, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Munne, S.
Right arrow Articles by Estop, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 6, No. 5, pp. 703-708, 1991
© 1991 European Society of Human Reproduction and Embryology

other

The effect of in-vitro ageing on mouse sperm chromosomes

Santiago Munne1,3 and Anna Estop1,2

1Department of Medical Genetics, West Penn Hospital 4800 Friendship Avenue, Pittsburgh, PA 15224 2Department of Human Genetics, University of Pittsburgh PA, USA

Correspondence: 3To whom correspondence should be addressed

In-vitro ageing has long been suspected to affect adversely the ability of spermatozoa to fertilize and produce viable zygotes. A comparison of the first cleavage chromosome complement of 824 mouse embryos fertilized with fresh spermatozoa and 656 embryos fertilized with spermatozoa aged for 12, 24 or 48 h is reported herein. The chromosome analysis of first-cleavage embryos allows us to study directly the paternal and maternal chromosome complements which contribute to the embryo. Both chromosome clusters remain separate when an antimitotic agent is used to prevent metaphase synchronization, allowing identification of maternal and paternal chromosomes. We show that after sperm ageing, there is a decrease in the fertilization rate from 75% in the controls to 57.5% (12 h of ageing), 63.5% (24 h of ageing) and 4.4% (48 h of ageinaj. Simultaneously, the level of chromosome structural abnormalities increases from 1.3% in the controls to 16.5% after 12 h of ageing, 18.8% after 24 h of ageing and 59% after 48 h of ageing. The incidence of aneuploidy and polyploidy is not affected by ageing of the spermatozoa. Most of the structural abnormalities are paternal in origin and are presumably induced by the ageing of spermatozoa.

Key words: IVF/sperm ageing/chromosome breakage


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. White, R. H. Wagner, F. Helfenstein, S. A. Hatch, H. Mulard, L. C. Naves, and E. Danchin
Multiple deleterious effects of experimentally aged sperm in a monogamous bird
PNAS, September 16, 2008; 105(37): 13947 - 13952.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Tateno and Y. Kamiguchi
Evaluation of Chromosomal Risk Following Intracytoplasmic Sperm Injection in the Mouse
Biol Reprod, August 1, 2007; 77(2): 336 - 342.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Watanabe
Frequent structural chromosome aberrations in immotile human sperm exposed to culture media
Hum. Reprod., April 1, 2004; 19(4): 940 - 947.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.