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Human Reproduction, Vol. 6, No. 6, pp. 867-871, 1991
© 1991 European Society of Human Reproduction and Embryology

other

Free radical scavengers ameliorate the 2-cell block in mouse embryo culture

Michael Legge1 and Martin H. Sellens2

Department of Biology, University of Essex Wivenhoe Park, Colchester CO4 3SQ, UK 1Present address:Department of Biochemistry, University of Otago PO Box 56, Dunedin, New Zealand

Correspondence: 2To whom correspondence should be addressed

Mouse zygotes from a strain combination which fails to undergo cleavage in vitro (the 2-cell block) were cultured in a variety of media, using either tow (5%) or normal (20%) oxygen tension in the gas phase. Development beyond the 4-cell stage was only achieved in medium containing serum and with normal oxygen tension. When serum-free medium was supplemented with free radical scavengers, reduced glutathione (1 mM), but not histidine, catalase or superoxide dismutase, development to the morula or blastocyst stage was promoted in 50% of zygotes cultured with normal oxygen tension. These data suggest that the 2-cell block is a consequence, at least in part, of free radical damage incurred by embryos during collection and culture, and that medium supplementation with the radical scavenger, reduced glutathione, can improve embryo developmentin vitro.

Key words: free radicals/embryo culture/2-cell block


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