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Human Reproduction, Vol. 6, No. 7, pp. 987-991, 1991
© 1991 European Society of Human Reproduction and Embryology

other

Protective role of superoxide dismutase in human sperm motifity: superoxide dismutase activity and lipid peroxide in human seminal plasma and spermatozoa

T. Kobayashi, T. Miyazaki1, M. Natori and S. Nozawa

Department of Obstetrics and Gynaecology, School of Medicine, Keio University Tokyo, Japan

Correspondence: 1To whom correspondence should be addressed at Department of Obstetrics and Gynaecology, Ogikubo Hospital. 3-1-24 Imagawa, Suginami, Tokyo 167, Japan

The levels of superoxide dismutase (SOD), a highly specific scavenging enzyme for superoxide anion radicals (O2–), and lipid peroxide produced by oxygen free radicals were measured in human seminal plasma and spermatozoa. Seminal plasma contained 366.8 ± 20.9 U/ml (mean ± SE) of SOD activity. SOD activity in human spermatozoa showed a significant correlation to the number of motile spermatozoa, while the activity in seminal plasma did not relate to the sperm concentration or motility. The lipid peroxide concentration in seminal plasma was 6.22 ± 0.46 nmol/ml and had no significant relationship to sperm concentration or motility. The malondialdehyde (MDA) concentration in spermatozoa was significantly related to the number of immotile spermatozoa. A decrease in the motility of spermatozoa incubated in medium without seminal plasma was observed after 120 min, while the MDA concentration of the spermatozoa increased. Addition of exogenous SOD (400 U/ml) to the sperm suspension significantly decreased this loss of motility and the increase of the MDA concentration. These data suggest a significant role for SOD in sperm motility. It seems that lipid peroxidation of human spermatozoa may cause loss of motility and that SOD may inhibit this lipid peroxidation. These results suggest that SOD may have a possible clinical application in the use of spermatozoa for in-vitro fertilization (IVE) or artificial insemination.

Key words: human spermatozoa/lipid peroxide/oxygen free radical/sperm motility/superoxide dismutase


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