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Human Reproduction, Vol. 7, No. 2, pp. 156-161, 1992
© 1992 European Society of Human Reproduction and Embryology

other

Isolation and purification of human endometrial stromal and glandular cells using immunomagnetic microspheres

S. Fernández-Shaw1, S.C. Shorter, C.E. Naish, D.H. Barlow and P.M. Starkey

University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital Headington, Oxford, OX3 9DU, UK

Correspondence: 1To whom correspondence should be addressed

Isolation of pure preparations of the different cell populations of human endometrium is a prerequisite for studies of in-vitro function. Sieving of dispersed endometrial cells, followed by adsorption onto immunomagnetic microspheres coated with antibody to Thy-1 was used to separate glandular and stromal cells. The purity of these cell populations was checked with antibodies to cytokeratin and Thy-1. The stromal cells were 98% pure and 90% viable, gland cells were 82% pure with 76% viability. The purified cells were able to proliferate in vitro as shown by thymidine incorporation.

Key words: culture/endometrium/human isolation/immunomagnetic microspheres


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