Human Reproduction, Vol. 7, No. 5, pp. 612-620, 1992
© 1992 European Society of Human Reproduction and Embryology
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Microbial colonization and sperm-mucus interaction: results in 1000 infertile couples*
Division of Gynaecological Endocrinology, Women's Hospital, University of Heidelberg 6900 Heidelberg, FRG 2Department of Microbiology and Hygiene University of Heidelberg 6900 Heidelberg, FRG 3Department of Dermatology, University of Heidelberg 6900 Heidelberg, FRG
Correspondence: 1To whom correspondence should be addressed
After screening a large series of infertile patients (n = 1000 couples), potentially pathogenic microorganisms were identified in genital secretions of the majority of couples. None of the patients displayed signs or symptoms of infection of the lower genital tract. In semen specimens and cervical swabs, mycoplasmas were found in 18 and 12%, potentially pathogenic aerobic bacteria in 50 and 31%, additionally commensal aerobes in 38% and 25%, respectively. The microbial pattern showed great variability with polymicrobial growth most common. The rate of cultures positive for potentially pathogenic anaerobes was markedly influenced by the transport conditions. In endocervical material, Herpes simplex virus (HSV) was identified in 4.5%. Yeasts were found in the posterior vaginal fornix in 10%. Concerning both partners, there were only 10 couples (1%) from whom microorganisms in genital secretions could not be isolated, not including lactobacilli in females. This high prevalence of microbes and the low number of leukocytes indicate colonization rather than infection. When the microbial findings from both partners were analysed with regard to the outcome of sperm-cervical mucus penetration testing in vitro, which was performed simultaneously, no significant correlation was found. Furthermore, microbial prevalence did not differ between couples with ’explained‘ and ’unexplained‘ infertility. The results of this study demonstrate that in patients without symptoms of genital tract infection, microbial colonization is of minor importance for sperm-mucus interaction and that extensive microbial screening should be preferentially performed in cases of poor sperm and/or mucus function.
Key words: infertility/microbial colonization/sperm-mucus interaction
*Presented at the 2nd Düsseldorf Symposium on Interactions in Reproductive Medicine, November 18–20, 1990. Prepared for publication by N.J.Alexander, G.Freundl and V.Insler.
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