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Human Reproduction, Vol. 7, No. 5, pp. 646-651, 1992
© 1992 European Society of Human Reproduction and Embryology

other

The acrosome reaction-inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content

Patricio Morales1, Miguel Llanos2, Gabriela Gutierrez3, Paulina Kohen3, Pilar Vigil and David Vantman3

Unit of Reproduction and Development, Faculty of Biological Sciences, P. Catholic University of Chile PO Box 114-D, Santiago, Chile 2Unit of Reproductive Biology, INTA, University of Chile, Hospital Clinico San Borja Arriaran Santiago, Chile 3Mother and Child Institute, University of Chile, Hospital Clinico San Borja Arriaran Santiago, Chile

Correspondence: 1To whom correspondence should be addressed

In this study, we have evaluated the relationship between the acrosome reaction-inducing activity of individual human follicular fluid samples and their steroid content. Eighteen samples of follicular fluid were obtained during egg retrieval in six patients undergoing assisted fertilization. Motile spermatozoa were incubated in modified Tyrode's medium (26 mg/ml bovine serum albumin) for 20 h at 1 x 107 cells/ml. In a single experiment, aliquots of a semen specimen were simultaneously treated with an aliquot of each follicular fluid sample. The percentage of acrosome reacted spermatozoa was determined using fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FTTC-PSA) lectin. The fluids were also analysed by radioimmunoassay to determine the levels of progesterone, 17{alpha}-hydroxy-progesterone, testosterone and oestradioh The results showed that there was a positive, highly significant correlation between the acrosome reaction-inducing activity and the progesterone level of each follicular fluid sample (r = 0.72, P < 0.005). Additionally, treatment of the follicular fluid samples with charcoal-dextran caused both a decrease in progesterone concentration and the total loss of the acrosome reaction-inducing activity. The addition of progesterone restored the acrosome reaction-inducing ability in 88% of samples. These data support the idea that progesterone in follicular fluid is the molecule responsible for inducing the acrosome reaction in human spermatozoa.

Key words: acrosome/fertilization/human spermatozoa/progesterone/steroids


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