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Human Reproduction, Vol. 7, No. 8, pp. 1094-1097, 1992
© 1992 European Society of Human Reproduction and Embryology

other

Insulin-like growth factor-I stimulates amino acid accumulation in cultured human granulosa cells

Torbjörn Hillensjö1, Christina Bergh and Ulrika Selleskog

Department of Obstetrics and Gynaecology, University of Göteborg, Sahlgrenska Hospital S-413 45 Göteborg, Sweden

Correspondence: 1To whom correspondence should be addressed

In order to study the effect of insulin-like growth factor (IGF)-I on amino acid transport, granulosa-luteal cells were obtained from stimulated cycles in women undergoing in-vitro fertilization. The cells were precultured for 1—3 days in serum-containing medium. The medium was then changed to serum-free with added IGF-I and/or human chorionic gonadotrophin (HCG). Following 24 h culture, the cellular uptake of [14C]{alpha}-aminoisobutyric acid AIB (0.1 mM, 0.6 mCi/ml) was studied during a 2–6 h incubation. The results showed that IGF-I (10–100 ng/ml) consistently stimulated AIB uptake to levels which were 44 ± 7% above the control (n = 11 experiments). The stimulatory effect of IGF-I was abolished by HCG and was reduced by IGF-binding protein. In conjunction with previous findings that IGF-I stimulates the uptake and incorporation of thymidine into DNA, these results suggest that IGF-I is involved in growth of human granulosa-luteal cells.

Key words: {alpha}-aminoisobutyric acid/granulosa-luteal cells/HCG/insulin-like growth factor I


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