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Human Reproduction, Vol. 8, No. 1, pp. 89-90, 1993
© 1993 European Society of Human Reproduction and Embryology

case-report

CASE REPORT: Pregnancy using spermatozoa aspirated from the vas deferens in a patient with ejaculatory failure due to spinal injury

A.V. Hirsh1,3, C. Mills1, S.L. Tan1,2, J. Bekir1 and P. Rainsbury1

1The Hallam Medical Centre 112 Harley Street, London WIN 1AF 2Department of Obstetrics and Gynaecology, King's College School of Medicine and Dentistry London, UK

Correspondence: 3To whom correspondence should be addressed

Aspiration of the vas deferens under local anaesthesia was carried out in a man with ejaculatory failure due to tetraplegia after rectal electrostimulation had failed to provide an ejaculate satisfactory for use in in-vitro fertilization. After passage through a discontinuous Percoll gradient and exposure to 3 mmol pentoxifylline and 3 mmol 2-deoxyadenosine, the final preparation consisted of 50 000 spermatozoa with 4% progressive motility. This was sufficient for the insemination of seven of the 23 oocytes that were collected from the patient's wife by transvaginal ultrasound-guided follicular aspiration. Three high-grade embryos were produced which were transferred transcervically into the uterus 2 days later. A singleton pregnancy resulted which is currently ongoing at 30 weeks gestation. Aspiration of the vas deferens is a relatively simple technique which provides an acceptable alternative to rectal electrostimulation, artificial sperm reservoirs and donor insemination in patients with ejaculatory failure.

Key words: ejaculation failure/in-vitro fertilization/male infertility/paraplegia/vas deferens


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