Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Parinaud, J.
Right arrow Articles by Grandjean, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parinaud, J.
Right arrow Articles by Grandjean, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 8, No. 10, pp. 1685-1688, 1993
© 1993 European Society of Human Reproduction and Embryology

research-article

Andrology: Comparison between fluorescent peanut agglutinin lectin and GB24 antibody techniques for the assessment of acrosomal status

J. Parinaud1,2,3, B. Labal1, G. Vieitez1,2, G. Richoilley1 and H. Grandjean2

1Centre de Fécondation In Vitro, CHU La Grave Place Lange, 31052 Toulouse Cede 2Institut National de la Santé et de la Recherche Médicale, Contrat Jeune Formation 89–08 (INSERM CJF 89-08), CHU La Grave Place Lange, 31052 Toulouse Cedex, France

Correspondence: 3To whom correspondence should be addressed

In order to compare fluorescent peanut (Arachis hypogaea) agglutinin lectin and GB24 antibody (specific for the inner acrosomal membrane) techniques for the assessment of acrosome reaction, both methods were applied on semen specimens obtained from patients undergoing in-vitro fertilization (IVF). The acrosome status was evaluated after a 4 h incubation in B2 medium with and without calcium ionophore A23187. Results obtained with both techniques were compared and studied as a function of IVF outcome. The percentage of spontaneous acrosome-reacted spermatozoa was higher when assessed by lectin than by GB24 (19 ± 2% versus 11 ± 1%; P < 0.001). The difference between the two methods [lectins minus GB24) was significantly higher in abnormal than in normal spermatozoa (10 ± 2% versus 4 ± 2%; P < 0.05), but did not significantly correlate with the percentage of acrosomes with abnormal morphology (r = 0.28; NS). When studied in relation to the IVF results, the response to A23187 was higher in successes than in failures (45 ± 2% versus 34 ± 4%; P < 0.05) but there was no significant difference between methods. Thus the assessment of acrosome reaction is strongly influenced by the method used, particularly in abnormal spermatozoa. Since the results obtained with lectins were higher in abnormal spermatozoa, GB24 seems to be more effective for assessment of true acrosome reaction.

Key words: acrosome reaction/GB24 antibody/lectins/semen quality/IVF


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
B.S. Jaiswal, M. Eisenbach, and I. Tur-Kaspa
Detection of partial and complete acrosome reaction in human spermatozoa: which inducers and probes to use?
Mol. Hum. Reprod., March 1, 1999; 5(3): 214 - 219.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.