Human Reproduction, Vol. 8, No. 12, pp. 2047-2051, 1993
© 1993 European Society of Human Reproduction and Embryology
review-article |
Endocrinology: Daily measurements and in-vitro effects of human chorionic gonadotrophin in the early luteal phase
Instituto Valenciano de Infertilidad and Department of Paediatrics, Obstetrics and Gynaecology, Valencia University School of Medicine Valencia, Spain
Correspondence: 1To whom correspondence should be addressed at: Instituto Valenciano de Infertilidad, Guardia Civil, 23, Valencia 46020, Spain
The purpose of the present study was to analyse daily measurements of human chorionic gonadotrophin (HCG) in in-vitro fertilization (IVF) cycles and to reproduce the effects of HCG in vitro using human granulosa—luteinized cells from the same patients. The study population consisted of nine women undergoing IVF because of tubal infertility in whom blood was drawn every 24 h from the day of the ovulatory dose of HCG (10 000 IU) until 6 days after ovum pick-up. Granulosa—luteal cells from the follicular aspirates were collected and cultured in vitro up to 6 days in the presence of increasing concentrations (0, 0.01, 0.1, 1.0 and 100.0 IU/ml) of HCG. Serum progesterone and HCG in vivo as well as progesterone accumulation in vitro on days 2, 4 and 6, were the main outcome measures. Maximum HCG concentrations (0.25 IU/ml) were reached the day before ovum pick-up, and continuously decreased until day 6 after ovum retrieval. HCG did not stimulate progesterone production in vitro at any dose tested until day 6 after ovum pick-up. Then, 0.01 IU/ml resulted significantly (P < 0.05) stimulatory compared to controls, while 1.0 IU/ml was inhibitory (P < 0.05). It is concluded that HCG supplementation in an IVF cycle is unnecessary until day 6 after ovum pick-up. On day 6, progesterone production is stimulated with very low concentrations of HCG.
Key words: early luteal phase/granulosa—luteal cells/HCG/IVF/progesterone release
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