Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Noto, V.
Right arrow Articles by Gordts, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noto, V.
Right arrow Articles by Gordts, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 8, No. 12, pp. 2115-2118, 1993
© 1993 European Society of Human Reproduction and Embryology

review-article

Fertilization and early embryology: Mitochondrial distribution after fast embryo freezing

Vincenzo Noto1,3, Rudi Campo1, Patricia Roziers1, Kristien Swinnen1, Miet Vercruyssen1 and Stephan Gordts1,2,4

1Medical Centre for Fertility Diagnostics and In Vitro Fertilization and Embryo Transfer Tiense Vest 168, 3000 Leuven 2University of Louvain, Cliniques Universitaires St-Luc Avenue Hippocrate 10, 1200 Brussels, Belgium

Correspondence: 4To whom correspondence should be addressed

Mitochondrial distribution pattern after ultrarapid freezing in dimethylsulphoxide was assessed in human multipronucleate zygotes, 2-cell and 4-cell stage embryos using rhodamine 123. The mitochondrial distribution pattern was evaluated at 37°C after a 30 min incubation in rhodamine 123 solution, 4 h and 24 h after thawing. Non-frozen human unfertilized oocytes, 2-cell and 4-cell embryos used as a control showed a homogeneous distribution of mitochondria throughout the cytoplasm, while there was sequestration of mitochondria from the cortex to the region surrounding the pronuclei in multipronucleate zygotes. Morphologically intact multipronucleate zygotes, 2-cell and 4-cell stage embryos after quick freeze—thawing showed the same mitochondrial distribution pattern found in the unfrozen controls. Mitochondria exhibited a typical severe aggregation (clumping) throughout the cytoplasm when non-viable single blastomeres or embryos at thawing were exposed to rhodamine 123. Our study indicates that quick freezing does not affect subcellular structures. The well-organized and specific mitochondrial distribution appeared still to be present after frozen storage, and subcellular structures seemed to be rather resistant targets for cryo-injury.

Key words: cryopreservation/mitochondria/ultrarapid

3Present address: Centro Sterilità, Casa di Cura ‘Città di Verona’, Lungadige Campagnola 5, 1-37126 Verona, Italy


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
T. E. Ludwig, J. M. Squirrell, A. C. Palmenberg, and B. D. Bavister
Relationship Between Development, Metabolism, and Mitochondrial Organization in 2-Cell Hamster Embryos in the Presence of Low Levels of Phosphate
Biol Reprod, December 1, 2001; 65(6): 1648 - 1654.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S.-P. Park, E. Y. Kim, J. H. Oh, H. K. Nam, K. S. Lee, S. Y. Park, E. M. Park, S. H. Yoon, K. S. Chung, and J. H. Lim
Ultra-rapid freezing of human multipronuclear zygotes using electron microscope grids
Hum. Reprod., August 1, 2000; 15(8): 1787 - 1790.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A.L. Mauri, C.G. Petersen, R.L.R. Baruffi, R.C. Ferreira, and J.G. Franco Jr
Clinical experience with ultrarapid cryopreservation of human embryos resulting from intracytoplasmic sperm injection: Brief communication
Hum. Reprod., September 1, 1999; 14(9): 2362 - 2366.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.