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Human Reproduction, Vol. 8, No. 12, pp. 2128-2132, 1993
© 1993 European Society of Human Reproduction and Embryology

review-article

Fertilization and early embryology: Determination of the acrosome reaction in human spermatozoa is predictive of fertilization in vitro

R Henkel1, C. Miiller, W. Miska, H. Gips2 and W.-B. Schill

Department of Dermatology and Andrology, Andrological Research Unit, Justus Liebig University Gaffkystrasse 14, 35385 Giessen 2Study Group of Reproductive Medicine, Department of Obstetrics and Gynaecology, Justus Liebig University Klinikstrasse 32, 35385 Giessen, Germany

Correspondence: 1To whom correspondence should be addressed

The acrosome reaction was determined in aliquots from ejaculates of 74 patients undergoing in-vitro fertilization at the University of Giessen, Germany, by means of the triple-stain technique. The percentage of acrosome-reacted spermatozoa after low-temperature induction of the acrosome reaction was not significantly related with the fertilization rate (H test, P = 0.693, SJ test, P = 0.366). However, all patients showing < 13.0% acrosome-reacted spermatozoa had poor fertilization rates. Highly significant differences between patients could be detected by correlating the inducibility of the acrosome reaction with the fertilization rate (H test, P = 0.018; SJ test, P = 0.004); patients with high fertilization rates showed a corresponding high inducibility of acrosome reactions. From our results, it is evident that percentages of acrosome-reacted spermatozoa < 13.0% or an inducibility of the acrosome reaction of <7.5% are indicative of subfertility.

Key words: acrosome reaction/diagnosis/fertilization/IVF/triple-stain


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