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Human Reproduction, Vol. 8, No. 12, pp. 2155-2166, 1993
© 1993 European Society of Human Reproduction and Embryology

review-article

Fertilization and early embryology: Fertilization potential in vitro is correlated with head-specific mannose — ligand receptor expression, acrosome status and membrane cholesterol content*

Susan Benoff1, Ian Hurley2, George W. Cooper, Francine S. Mandel3, Avner Hershlag, Gerald M. Scholl and David L. Rosenfeld

Department of Obstetrics and Gynecology, North Shore University Hospital, and Department of Obstetrics and Gynecology, Cornell University Medical College 33 Walt Whitman Road, Huntington Station, NY 11746, USA 2Department of Obstetrics and Gynecology, North Shore University Hospital, Kemron Environmental Services 33 Walt Whitman Road, Huntington Station, NY 11746 3Department of Research, North Shore University Hospital and Department of Public Health, Cornell University Medical College New York, USA

Correspondence: 1To whom correspondence should be addressed at: Molecular Biology Laboratory, Division of Human Reproduction, Department of Ob/Gyn, North Shore University Hospital, 300 Community Drive, Boas-Marks, Biomedical Science Research Center, Room 125, Manhasset, NY 11030, USA

Normozoospermic men who do not fertilize human eggs in vitro constitute a clearly identifiable class of occult male factor infertility. We have studied the relationships between in-vitro fertilization (IVF) outcomes and the appearance of sperm surface mannose lectin/zona pellucida ligand activity measured by the binding of fluorescein isothiocyanate (FITC)-labelled mannosylated albumin. Of 338 semen specimens with normal profiles, 10 (2.96%) failed to fertilize in vitro (IVF–). Motile spermatozoa from six of these were analysed retrospectively for zona ligand binding activity on the sperm head in relation to acrosome status and the free cholesterol content of their plasma membrane. IVF+ males and fertile donors served as controls. The six IVF– occult male factor cases were distinguished from controls by their failure to show time-dependent increases in the percentage of spermatozoa exhibiting head-directed mannose ligand activity, by the slow loss or apparent increase in membrane cholesterol and by the absence of spontaneous and/or mannose-induced acrosome exocytosis.

Key words: capacitation/human spermatozoa/in-vitro fertilization/mannose-specific receptor/neoglycoprotein ligands

*Presented in part at the 48th Annual Meeting of the American Fertility Society, New Orleans, LA, November 2–5, 1992 (Abstract P-192).


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