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Human Reproduction, Vol. 8, No. 5, pp. 678-683, 1993
© 1993 European Society of Human Reproduction and Embryology

other

In-vitro synthesis of total protein and placental protein PP14 by the Fallopian tube mucosa: variation in relation to anatomical site, the ovarian cycle and the menopause

S.D. Maguiness1, K. Shrimanker1, O. Djahanbakhch1, J.J. Deeks3, B. Teisner2 and J.G. Grudzinskas1,4

1Academic Unit of Obstetrics and Gynaecology, The London Hospital Medical College, The Royal London Hospital Trust Whitechapel, London E1 1BB Newham General Hospital Glen Road, Plaistow, London E13 8RU, UK 2Institute of Medical Microbiology, Odense University Denmark 3Department of Epidemiology and Medical Statistics, The London Hospital Medical College at QMW Mile End Road, London El 4NS, UK

Correspondence: 4To whom correspondence should be addressed at: Academic Unit of Obstetrics and Gynaecology, The London Hospital Medical College, The Royal London Hospital Trust, Whitechapel, London El 1BB, UK

De-novo synthesis and secretion of protein by short term explants of mucosa from each anatomical section of the Fallopian tube and endometrium of pre-menopausal (n = 25) and tubal mucosa of post-menopausal (n = 5) women were studied by demonstration of incorporation of radiolabelled L-[35S]methionine and one-dimensional SDS — polyacrylamide gel electrophoresis. A consistent finding in 25 pre-menopausal women was the presence of a 25 kDa protein band synthesized by tissue obtained throughout the ovarian cycle. Western blotting demonstrated that this protein band contained placental protein 14 (PP14)-like immunoreactivity in the proliferative (n = 2) and luteal phase (n = 2) of the ovarian cycle. To determine if there is quantitative variation in total protein and PP14 synthesis and secretion during the ovarian cycle, the total quantities of protein and PP14 synthesized were determined by Coomassie Brilliant Blue staining and radioimmunoassay respectively. Analysis of the results of total protein assay revealed statistically significant differences in relation to the anatomical origin of the study tissue (P < 0.01), the stage of the ovarian cycle (P < 0.04) and the manner in which each anatomical site varied during the ovarian cycle (P < 0.01), the endometrium being significantly different from the Fallopian tube. When the data for PP14 synthesized by the Fallopian tube mucosa were analysed, these effects were not seen. PP14 was not detected in the culture media of Fallopian tube mucosa obtained from post-menopausal women.

Key words: Fallopian tube/protein synthesis/PP14


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