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Human Reproduction, Vol. 8, No. 5, pp. 691-694, 1993
© 1993 European Society of Human Reproduction and Embryology

other

Effects of epidermal growth factor in the final stages of nuclear and cytoplasmic oocyte maturation in humans

E. Gómez, M.J. de los Santos, A. Ruiz, J.J. Tarín, J. Remohí and A. Pellicer1

Instituto Valenciano de Infertilidad, and Department of Paediatrics, Obstetrics and Gynaecology, Valencia University School of Medicine Valencia, Spain

Correspondence: 1To whom correspondence should be addressed at: Instituto Valenciano de Infertilidad, Guardia Civil, 23, Valencia-46020, Spain

Evidence has accumulated in mammals suggesting a positive role for epidermal growth factor (EGF) as an inducer of oocyte maturation. The potential use of EGF as inducer of cytoplasmic and nuclear maturation was tested in women with > 10 oocytes retrieved in in-vitro fertilization (IVF), since we have previously observed that such oocytes are immature. Oocytes from 17 high responders were randomly allocated to one of the three treatment groups upon retrieval: control receiving no EGF (n = 93), 1.0 ng/ml EGF (n = 92) and 10.0 ng/ml EGF (n = 77) for 6 h before insemination. The rates of fertilization were respectively 54.6, 59.0, and 46.1%, suggesting that EGF is not effective at this maturational stage after this length of exposure. Embryo development was further analysed by the appearance of the embryos under the dissecting microscope and the number of blastomeres developed 48 h after insemination. No difference between groups was observed considering the number of blastomeres developed. However, embryos derived from oocytes treated with 10 ng/ml EGF displayed a worse appearance under the microscope. It is concluded that a 6 h incubation with EGF does not seem to affect cytoplasmic maturation in oocytes obtained after gonadotrophin treatment, as ascertained by the rate of fertilization following oocyte insemination.

Key words: cytoplasmic and nuclear maturation/EGF/IVF/oocytes


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