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Human Reproduction, Vol. 8, No. 8, pp. 1199-1202, 1993
© 1993 European Society of Human Reproduction and Embryology

research-article

Immunology: Evidence for the in-vitro de-novo synthesis of immunoglobulin and a previously undescribed 17 kDa protein (TEP-2) by the mucosa of the Fallopian tube

S.D. Maguiness2, K. Shrimanker1, O. Djahanbakhch2, B. Teisner3 and J.G. Grudzinskas1,4

1Academic Unit of Obstetrics and Gynaecology, The London Hospital Medical College, The Royal London Hospital Trust Whitechapel, London E1 1BB 2Newham, General Hospital Glen Road, Plaistow, London, UK 3Institute of Medical Microbiology, Odense University Odense, Denmark

Correspondence: 4To whom correspondence should be sent at: Academic Unit of Obstetrics and Gynaecology, The London Hospital Medical College, The Royal London Hospital Trust, Whitechapel, London El 1BB, UK

De-novo synthesis and secretion of proteins by short-term explants of matched Fallopian tube mucosa and endometrium were studied using radiolabelled L-[35S]methionine and [3H]glucosamine. To compare directly each anatomical site of the Fallopian tube and endometrium from the same source, newly synthesized proteins were separated on one-dimensional polyacrylamide gel electrophoresis and examined by autoradiography. De-novo synthesis of two protein bands provisionally designated as tubal epithelial protein 1 (TEP-1) and tubal epithelial protein 2 (TEP-2), was observed in explants of the Fallopian tube mucosa obtained from each anatomical site throughout the ovarian cycle (n = 20). TEP-2 was not apparent in tubal mucosa obtained from post-menopausal women (n = 5). De-novo synthesis of TEP-1 and TEP-2 was not apparent in autoradiographs of radiolabelled proteins from short-term explants of endometrium. From the autoradiographs the molecular mass of TEP-1 and TEP-2 was calculated to be 25 kDa and 17 kDa, respectively. Incorporation of glucosamine into newly synthesized protein occurred in TEP-2 but not TEP-1. TEP-1 was observed to be immuno-chemically identical to immunoglobulin x light chains.

Key words: endometrium/Fallopian tube/immunoglobulins/mucosa/tubal epithelial proteins


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