Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Jaroudi, K.A.
Right arrow Articles by Sheth, K.V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jaroudi, K.A.
Right arrow Articles by Sheth, K.V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 8, No. 9, pp. 1438-1442, 1993
© 1993 European Society of Human Reproduction and Embryology

research-article

Andrology: Percoll semen preparation enhances human oocyte fertilization in male-factor infertility as shown by a randomized cross-over study

K.A. Jaroudi1, J.A. Carver-Ward2, C.J.C.M. Hamilton3, U.V. Sieck and K.V. Sheth2

Department of Obstetrics and Gynaecology King Faisal Specialist Hospital and Research Centre P.O. Box 3354, Riyadh 11211, Saudi Arabia 2Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre P.O. Box 3354, Riyadh 11211, Saudi Arabia 3Department of Obstetrics and Gynaecology, Nijmegen University P.O. Box 9101, 6500 HB Nijmegen, The Netherlands

Correspondence: 1To whom correspondence should be addressed

The aim of this study was to compare two methods of semen preparation: multiple tube swim-up and Percoll separation, using a randomized cross-over clinical study, in which sperm parameters, oocyte fertilization rates, embryo quality and cell stage were analysed. Overall, there was no difference between the two preparation methods in the normozoospermic cycles. In the male-factor cycles, Percoll extracted a higher total number of spermatozoa (P = 0.02), increased the concentration of motile spermatozoa (P = 0.02), increased the total number of motile spermatozoa per sample (P = 0.02), and enhanced the recovery rate of motile spermatozoa (P = 0.04) compared to swim-up. There was a significant improvement in fertilization rates (P = 0.0006), in the percentage of embryos over 2-cell stage on day of transfer (P = 0.004), and in the number of replaced embryos per transfer (P = 0.01) in the Percoll as compared to swim-up cycles. There was no significant difference in embryo quality. We conclude, therefore, that in advanced reproductive procedures where sperm dysfunction exists, semen preparation with Percoll should replace the swim-up technique.

Key words: in-vitro fertilization/Percoll gradient centrifugation/semen preparation/swim-up


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.