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Human Reproduction, Vol. 8, No. 9, pp. 1443-1447, 1993
© 1993 European Society of Human Reproduction and Embryology

research-article

Andrology: Effects of platelet activating factor on human sperm function in vitro

K. Sengoku1, K. Tamate, Y. Takaoka and M. Ishikawa

Department of Obstetrics and Gynaecology, Asahikawa Medical College Nishikagura 4 Sen 5 Gou, Asahikawa, Japan 078

Correspondence: 1To whom correspondence should be addressed

The direct effects of platelet activating factor (PAF) and the specific PAF receptor antagonist, CV-3988, on the fertilizing ability of human spermatozoa were investigated. PAF (10–7–10–11 M) increased the human sperm penetration rates in a sperm penetration assay at all doses >10–11 M. In contrast, treatment of the spermatozoa with 10–5 CV-3988 caused a significant decrease in human sperm penetration of zona-free hamster oocytes and adversely affected sperm motility after 24 h of incubation. This suppression was reversed by the addition of PAF. The acrosome reaction was also enhanced by PAF treatment of spermatozoa but this effect was not observed in calcium-free medium. While 10–5 M CV-3988 decreased the acrosome reaction, the inhibition was also reversed by the addition of PAF. These results suggest that PAF may have a direct role in the fertilizing capacity of human spermatozoa. These findings also suggest that PAF may have a clinical application in an in-vitro fertilization programme.

Key words: acrosome reaction/human spermatozoa/platelet activating factor/sperm penetration assay


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