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Human Reproduction, Vol. 8, No. 9, pp. 1455-1458, 1993
© 1993 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryology: Co-culture with granulosa cells does not increase the fertilization rate in couples with previous fertilization failures

M. Plachot1, J. Mandelbaum, A.M. Junca, J.M. Antoine2, J. Salat-Baroux2 and J. Cohen3

U173 INSERM, Hôpital Necker 149 rue de Sèvres, 75015 Paris 2Maternité Guy Le Lorier, Hôpital Tenon 4, rue de la Chine, 75020 Paris 3Hôpital de Sèvres 141, Grande rue 92310 Sèvres, France

Correspondence: 1To whom correspondence should be addressed

Ten couples included in our in-vitro fertilization programme, selected because of one or more previous total fertilization failures or a low fertilization rate (<20%), were entered in an experimental protocol of egg insemination on autologous granulosa cells. Half the oocytes from each patient were randomly assigned to either a control or a co-culture group. We observed no difference in the fertilization rate between the control (16.2%) and co-culture groups (12.1%). Only two couples benefited from this technique since fertilization was obtained only in co-culture. The poor efficiency of this protocol led us to propose the use of sperm micro-injection if all classical attempts to improve fertilization were unsuccessful.

Key words: co-culture/granulosa cells/in-vitro fertilization/male infertility


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