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Human Reproduction, Vol. 8, No. suppl_2, pp. 133-137, 1993
© 1993 European Society of Human Reproduction and Embryology

Quantitative two-site enzyme-linked immunosorbent assays for inhibin A, activin A and activin B

T. Woodruff1,4, L. Krummen1, D. Baly2, S. Garg2, D. Allison2, M. Sadick2, W. Wong2, J. Mather1 and M. Soules3

1 Department of Cell Culture and Fermentation, Research and Development 460 Pt San Bruno Blvd, South San Francisco, CA 94080 2 Department of Medicinal and Analytical Chemistry, Genentech, Inc. 460 Pt San Bruno Blvd, South San Francisco, CA 94080 3 Department of Obstetrics and Gynecology, University of Washington Seattle, WA, USA

Correspondence: 4To whom correspondence should be addressed

We have developed three specific enzyme-linked immunosorbent assay (ELISA) formats which quantitate inhibin A in conditioned media and serum. The assays are sensitive in a range 0.078–5.0 ng/ml and have been characterized in terms of cross-reactivity to inhibin related proteins. The CK:CK assay format recognizes inhibin A, inhibin B and inhibin-related molecules, while the 9A9:CK assay format recognizes inhibin A and inhibin A precursors, but not free {alpha}-subunit. The 11B5:CK assay appears to recognize only mature 32 kDa inhibin A. Additionally, we have developed separate, specific ELISA formats which quantitate activin A and activin B. The assays have a range of 0.2–50 ng/ml and 0.4–50 ng/ml for activin A and recombinant activin B, respectively. These assays are presently being used to examine the concentration of inhibin A, activin A and activin B in clinical serum samples.

Key words: activin/bioassay/ELISA/inhibin/quantitation


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