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Human Reproduction, Vol. 9, No. 4, pp. 604-609, 1994
© 1994 European Society of Human Reproduction and Embryology

other

Characterization of follicular energy metabolism

N.I. Boland1,, P.G. Humpherson2, H.J. Leese2 and R.G. Gosden

Department of Physiology, University of Edinburgh Medical School Teviot Place, Edinburgh EH8 9AG, UK 2Department of Biology, University of York York YOI 5DD, UK

Correspondence: 1To whom correspondence should be addressed

It has been shown that mouse ovarian follicles have a large glycolytic capacity, and this study was undertaken to determine whether follicles can develop normally using glycolysis alone. Pre-antral mouse follicles were grown using an in-vitro system which supports development to the pre-ovulatory stage within 5 days. Cultures were maintained in either aerobic conditions in the presence of the inhibitor of oxidative phosphorylation, sodium malonate, or under anaerobic conditions. Samples of media were removed every 24 h and analysed for oestradiol using an enzyme-linked immunosorbent assay (ELISA) technique and for lactate and glucose using a fluorometric assay. Follicle size, oestradiol production and glycolytic rate were not significantly different between control and sodium malonate-treated follicles. Follicles cultured under anaerobic conditions showed significantly slower rates of growth and oestradiol production compared with controls. However, the rate of glycolysis was significantly higher during anoxia. Results indicated that anaerobic glycolysis may sustain limited periods of growth during the pre-antral phase, but that the presence of oxygen is vital to ensure normal development. It is concluded that pre-antral follicles can undergo development to the pre-ovulatory stage using glycolysis alone, a feature which may allow them to conserve their limited supply of oxygen for other vital biosynthetic processes.

Key words: follicle/glycolysis/malonate/ovary/TCA cycle


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