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Human Reproduction, Vol. 9, No. 7, pp. 1265-1270, 1994
© 1994 European Society of Human Reproduction and Embryology

research-article

Genetics: Discontinuous Percoll gradients enrich X-bearing human spermatozoa: a study using double-label fluorescence int-situ hybridization

Huai-Xiu Wang1, Sean P. Flaherty2, Nicholas J. Swann and Colin D. Matthews

Department of Obstetrics and Gynaecology, The University of Adelaide, The Queen Elizabeth Hospital Woodville South Australia 5011, Australia

Correspondence: 2To whom correspondence should be addressed

The aim of this study was to evaluate the efficacy of enriching X-bearing human spermatozoa using 12-step (25–80%) discontinuous Percoll gradients. X- and Y-bearing spermatozoa were simultaneously identified in neat semen (controls) and in 80% Percoll fractions from the same samples using double-label fluorescence in-situ hybridization and chromosome-specific DNA probes. Hybridization and labelling efficiencies of 95–99% were obtained in all samples. The mean ratio of X- to Y-bearing spermatozoa in the controls was 49.0: 48.2, whereas there was a significant enrichment (P < 0.0001) of X-bearing spermatozoa in the Percoll fractions (mean X:Y ratio was 55.1:41.1 or 1.35). The ratio varied from 1.1–1.5 in individual Percoll samples. There were no significant differences in the proportions of aneuploid spermatozoa (XX, YY, XY) between the control and Percoll fractions. We conclude that 12-step discontinuous Percoll gradients enrich X-bearing spermatozoa, but the degree of enrichment is insufficient for use in preconceptional sex selection.

Key words: human spermatozoa/fluorescence in-situ hybridization/Percoll/sex selection

1Present address: Shanxi Provincial People's Hospital, Taiyuan, People's Republic of China


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[Abstract] [Full Text] [PDF]


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