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Human Reproduction, Vol. 9, No. 7, pp. 1276-1280, 1994
© 1994 European Society of Human Reproduction and Embryology

research-article

Andrology: Co-culture with Vero cell monolayer maintains the motility of asthenozoospermic semen samples

Hsin-Fu Chen, Hong-Nerng Ho, Shee-Uan Chen, Yih-Ron Lien, Kuang-Han Chao, Heng-Ru Lin, Su-Cheng Huang, Tzu-Yao Lee and Yu-Shih Yang1

Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan

Correspondence: 1To whom correspondence should be addressed at: No. 7 Chung-Shan South Road, National Taiwan University Hospital, Taipei, Taiwan

The clinical effectiveness of co-culture with Vero (Green monkey kidney) cell monolayer in maintaining the motility and viability of fresh asthenozoospermic semen (18 samples) and frozen–thawed semen with poor motility (motility fraction <50%) (15 samples) in a 24-h period was evaluated. Co-culture with Vero cell monolayer in human tubal fluid (HTF) medium for 24 h resulted in a statistically better maintenance of motility percentage (P < 0.005), mean amplitude of lateral head displacement (ALH) (P < 0.005), and mean track speed (VCL) (P < 0.05) than culture in HTF medium alone. However, these motility parameters (motility percentage, ALH, VCL) declined soon after removal of spermatozoa from the monolayer. Co-culture with Vero cell monolayer also maintained the viability percentage of these sperm samples (52% of the original value) after the 24-h period compared with culture in HTF medium alone (22% of the original) (52% versus 22%, P < 0.05).It is concluded that Vero cell monolayer is effective in the maintenance of motility and viability of asthenozoospermic semen or frozen–thawed semen with poor motility.This co-culture system may be beneficial in enhancing the in-vitro performance of asthenozoospermic semen samples in the practice of assisted reproductive technology.However, its safety needs further evaluation.

Key words: asthenozoospermic semen/co-culture/computer-aided sperm analysis/Vero cell


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