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Hum. Reprod. Advance Access published online on April 21, 2007
Human Reproduction, doi:10.1093/humrep/dem096
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© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Vaginoplasty using autologous in vitro cultured vaginal tissue in a patient with Mayer–von-Rokitansky–Küster–Hauser syndrome

Pierluigi Benedetti Panici1,3, Filippo Bellati1, Terenzio Boni1, Federica Francescangeli2, Luigi Frati2 and Cinzia Marchese2

1 Department of Gynaecology, Obstetrics and Perinatology, University of Rome "La Sapienza", V.le Regina Elena, 324, 00161 Rome, Italy 2 Department of Experimental Medicine, University Sapienza, V.le Regina Elena, 324, 00161 Rome, Italy

3 Correspondence address. Tel: +39-3483410486; Fax: +39-06-49972615; E-mail: pierluigi.benedettipanici{at}uniroma1.it

Mayer–von-Rokitansky–Küster–Hauser syndrome (MRKHS) is characterized by vaginal agenesis with variable Müllerian duct abnormalities. The Abbè–McIndoe technique is considered a valid treatment option for vaginoplasty but no consensus has been reached on what material should be used for the neovagina canal wall lining. We report the first case of autologous vaginal tissue transplantation in a 28-year-old women with MRKHS. The patient was subjected to a 1 cm2 full-thickness mucosal biopsy from the vaginal vestibule. Following enzymatic dissociation, cells were inoculated onto collagen IV-coated plates and cultured for 2 weeks. The patient was subjected to a vaginoplasty with a modified Abbè–McIndoe vaginoplasty with 314 cm2 autologous in vitro cultured vaginal tissue for the canal lining. At 1 month from surgery, the vagina appeared normal in length and depth and a vaginal biopsy revealed normal vaginal tissue. The use of autologous in vitro cultured vaginal tissue to create a neovagina appears as an easy, minimally invasive and useful method.

Key words: autologous transplant/Mayer–von-Rokitansky–Küster–Hauser syndrome/vaginoplasty

Submitted on November 9, 2006; resubmitted on January 30, 2007; accepted on February 5, 2007.


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