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Hum. Reprod. Advance Access published online on May 12, 2008
Human Reproduction, doi:10.1093/humrep/den119
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration

L. Parmegiani1,3, G.E. Cognigni1, S. Bernardi1, W. Ciampaglia1, F. Infante1, P. Pocognoli1, C. Tabarelli de Fatis1, E. Troilo1 and M. Filicori1,2

1 Reproductive Medicine Unit, GynePro Medical Centers, Via Tranquillo Cremona 8, 40137 Bologna, Italy 2 Reproductive Endocrinology Center, University of Bologna, Bologna, Italy

3 Correspondence address. E-mail: l.parmegiani{at}gynepro.it or drlparmegiani{at}hotmail.com

BACKGROUND: A number of factors influence the success of oocyte cryopreservation and subsequent ICSI. The aim of the present study is to establish the ideal time, after oocyte retrieval, for human oocyte cryopreservation via slow freezing/rapid thawing protocol with 0.3 M sucrose concentration in cryoprotectant solution (SF/RT 0.3 M).

METHODS: Retrospective study with 75 patients on the clinical outcome of 93 oocyte thawing cycles divided into three groups. Group A: freezing within 2 h from oocyte retrieval. Group B: freezing between 2 and 3 h from retrieval. Group C: freezing after 3 h.

RESULTS: The rate of best quality embryos was significantly higher (35.2%; P = 0.050) in Group A than in Group C (14.1%). Pregnancy and implantation rates were 39.1% (9/23) and 18.5% (10/54) in Group A. Nine clinical pregnancies per 124 thawed (7.3%) and 73 injected (12.3%) oocytes were observed in Group A versus 3 pregnancies per 174 thawed, 103 injected (1.7%, 2.9%, P = 0.046 and 0.0049) in Group B and 4 per 139 thawed, 88 injected (2.9%, 4.5%, NS) in Group C. The overall yield from oocytes cryopreserved within 2 h of retrieval was 8.1 implantations per 100 oocytes thawed.

CONCLUSIONS: Embryo quality and clinical outcome of thawing cycles were significantly improved when oocyte cryopreservation by SF/RT 0.3 M was carried out within 2 h from oocyte retrieval.

Key words: human oocyte cryopreservation/slow freezing/high sucrose concentration/embryo quality

Submitted on December 21, 2007; resubmitted on February 15, 2008; accepted on March 18, 2008.


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