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Hum. Reprod. Advance Access published online on December 3, 2008
Human Reproduction, doi:10.1093/humrep/den434
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Effect of L-dopa on interleukin-1β-induced suppression of luteinizing hormone secretion in intact female rats

M.P. Sirivelu1, A.C. Shin2, G.I. Perez1,3, P.S. MohanKumar1,2,4 and S.M.J. MohanKumar1,2,5,6

1 Comparative Medicine and Integrative Biology, College of Veterinary Medicine, Michigan State University, B-440 Life Sciences Building, East Lansing, MI 48824, USA 2 Neuroscience Graduate Programs, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA 3 Department of Physiology, College of Veterinary Medicine, Michigan State University, B-440 Life Sciences Building, East Lansing, MI 48824, USA 4 Department of Pathobiology & Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, B-440 Life Sciences Building, East Lansing, MI 48824, USA 5 Department of Pharmacology and Toxicology, College of Veterinary Medicine, Michigan State University, B-440 Life Sciences Building, East Lansing, MI 48824, USA

6 Correspondence address. Tel: +1-517-432-2876; Fax: +1-517-353-8915; E-mail: mohankumrs{at}cvm.msu.edu

BACKGROUND: The cytokine, interleukin-1β (IL-1β), increases during immune stress and is known to suppress the preovulatory luteinizing hormone (LH) surge in female rats by decreasing hypothalamic norepinephrine (NE). We hypothesized that IL-1β could produce this effect by decreasing NE biosynthesis.

METHODS: Female Sprague–Dawley rats were implanted with a push–pull cannula in the medial preoptic area (MPA) of the hypothalamus and a catheter in the jugular vein. They were treated i.p. with the vehicle or 5 µg of IL-1β, the NE precursor, L-dopa, or a combination of L-dopa and IL-1β at 1300 hours on the day of proestrus. They were subjected to push–pull perfusion and serial blood sampling. Perfusates were analyzed for NE levels and serum samples for LH.

RESULTS: IL-1β treatment blocked the increase in NE levels in the MPA and the LH surge. Treatment with L-dopa was able to partially restore both NE and LH levels during the afternoon of proestrus. IL-1β treatment caused failure of ovulation and this effect was also reversed by L-dopa.

CONCLUSIONS: These results suggest that IL-1β could decrease NE levels in the MPA to suppress reproductive functions and L-dopa can be used to counter this effect.

Key words: IL-1β/hypothalamus/luteinizing hormone/L-dopa

Submitted on June 18, 2008; resubmitted on October 24, 2008; accepted on November 5, 2008.


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