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Hum. Reprod. Advance Access published online on December 18, 2008
Human Reproduction, doi:10.1093/humrep/den447
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed: the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given: if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative word this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

Helen F. Irving-Rodgers1, Stephanie Morris1, Rachael A. Collett1, Teija T. Peura2,5, Margaret Davy3, Jeremy G. Thompson1, Helen D. Mason4 and Raymond J. Rodgers1,6

1 Research Centre for Reproductive Health, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, Australia 2 South Australian Research and Development Institute, Turretfield Research Centre, Rosedale, South Australia 5350, Australia 3 Gynaecological Oncology, Royal Adelaide Hospital, South Australia, Australia 4 Basic Medical Sciences and Clinical Developmental Sciences, St. George's, University of London, London SW17 0RE, UK 5 Present address: Australian Stem Cell Centre, University of Queensland, Brisbane QLD 4072, Australia

6 Correspondence address. Tel: +61 883033932; Fax: +61 883034099; E-mail: ray.rodgers{at}adelaide.edu.au

BACKGROUND: The ovarian follicular basal lamina underlies the epithelial membrana granulosa and maintains the avascular intra-follicular compartment. Additional layers of basal lamina occur in a number of pathologies, including pili annulati and diabetes. We previously found additional layers of follicular basal lamina in a significant percentage of healthy bovine follicles. We wished to determine if this phenomenon existed in humans, and if it was related to oocyte function in the bovine.

METHODS AND RESULTS: We examined follicles from human ovaries (n = 18) by electron microscopy and found that many follicles had additional layers of basal lamina. Oocytes (n = 222) from bovine follicles with normal or unusual basal laminas were isolated and their ability to undergo in vitro maturation, fertilization and culture to blastocyst was compared. Healthy bovine follicles with a single layer of basal lamina had oocytes with significantly (P < 0.01) greater developmental competence than healthy follicles with additional layers of follicular basal lamina (65% versus 28%).

CONCLUSIONS: These findings provide direct evidence that the phenotype of the follicular basal lamina is related to oocyte competence.

Key words: ovary/basal lamina/oocyte competence/in vitro production

Submitted on April 22, 2008; resubmitted on November 11, 2008; accepted on November 15, 2008.


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