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Hum. Reprod. Advance Access published online on April 20, 2009
Human Reproduction, doi:10.1093/humrep/dep092
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© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Centromere-specific multicolour fluorescence in situ hybridization on human spermatocyte I and II metaphases

L. Uroz1, T. Liehr2, K. Mrasek2 and C. Templado1,3

1 Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain 2 Jena University Hospital, Institute of Human Genetics and Anthropology, Kollegiengasse 10, 07743 Jena, Germany

3 Correspondence address. Tel: +34-935811905; Fax: +34-935811025; E-mail: cristina.templado{at}uab.es

BACKGROUND: Most meiotic studies in metaphase spermatocytes have been carried out with classic cytogenetic techniques. The aim of this work was to adjust the centromere-specific multicolour fluorescence in situ hybridization (cenM-FISH) procedure to spermatocyte metaphases I and II in order to improve the identification of meiotic chromosome abnormalities.

METHODS: A total of 168 spermatocytes I and 66 spermatocytes II from two fertile males have been studied using cenM-FISH.

RESULTS: The mean frequency of meiotic abnormalities (synaptic, numerical and structural errors) found in metaphases I and II was 22.1 and 3.0%, respectively. The cenM-FISH technique has not only enabled the individual identification of chromosomes involved in meiotic disorders, but also increased the number of analysable cells, principally at metaphase II stage.

CONCLUSIONS: CenM-FISH is a useful tool to study the meiotic chromosomal disorders and mechanisms leading to chromosomally abnormal spermatozoa.

Key words: meiosis/centromere-specific multicolour fluorescence in situ hybridization/human spermatocytes/metaphase I/metaphase II

Submitted on February 2, 2009; resubmitted on March 20, 2009; accepted on March 24, 2009.


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