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Human Reproduction, Vol. 14, No. 8, 1927-1939, August 1999
© 1999 European Society of Human Reproduction and Embryology

Debates

Manipulating the genome to study reproduction

Mice with `humanized' zonae pellucidae

Philip.E. Castle1,1 and Jurrien Dean

1 Laboratory of Cellular and Developmental Biology, NIDDK, Building 6, Room B 1–26,National Institutes of Health, Bethesda, MD 20892, USA

The current revolution in biology has provided new insights into mechanisms of mammalianprocreation. However, detailed knowledge of the molecular basis of human reproduction remainslimited because of ethical, regulatory, and societal considerations. Although non-human primates are closely related and can serve as valuable models of human biology, cost and care of primate colonies are formidable and beyond the means of many investigators. Thus, development of small animal models that more closely approximate human reproduction is highly desirable.

Human genes can be introduced into mice using a variety of methodologies (Cohen-Tannoundji and Babinet, 1999Go). One approach is to establish homozygous `knockout' mouse lines from embryonic stem cells in which the gene of interest has been inactivated by insertional mutagenesis. This line can be crossed with a second transgenic line that expresses the homologous human protein. The flexibility of this approach lies in the ability to rescue the `null' phenotype with either . . . [Full Text of this Article]

Molecular basis of mammalian fertilization

Immunocontraceptive development

Autoimmune oophoritis and premature ovarian failure

Targeting pharmacological agents to the ovary

Future directions

Notes

References


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