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Figure 1. Stages of embryonic development observed in both in-vitro systems; (A) day 4 compacting embryo on Vero cells + S2 medium; (B) day 4 compacted embryo in S2 medium only; (C) day 4 early cavitating embryo in S2 medium only (blastocele just appearing); (D) day 5 late cavitating embryo on Vero cells + S2 medium [blastocele larger but no distinct inner cell mass (ICM) and trophectoderm (TE) laid down as yet]; (E) day 5 early blastocyst on Vero cells +S2 medium (ICM and TE laid down and embryo diameter usually similar or slightly larger than late cavitating embryo); (F) day 5 expanding blastocyst in S2 medium only (diameter not fully expanded as yet). Scale bar = 0.03 mm (all panels).
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